Plant Tissue Cult. & Biotech. 15(2): 113-119, 2005 (December)

Regeneration of Hydnocarpus kurzi (King) Warb. - A Red-listed Medicinal Plant

Shubhra Sinha, A.K.M. Sayeed Hassan and Shyamal K. Roy

Department of Botany, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh

Key words: Hydnocarpus kurzi, Medicinal plant, Shoot proliferation, Regeneration

Abastract

About 85% of the cultures regenerated showing four shoots per culture when explants of apical and axillary buds of young sprouts from naturally grown Hydnocarpus kurzi were cultured in MS supplemented with 2.5 mg/l BA + 0.5 mg/l NAA. Repeated subcultures, in the same medium, resulted in rapid shoot multiplication with eight shoots per culture. Addition of 15% (v/v) coconut water to the medium increased the number of shoot(s) up to 15 per culture. In vitro raised shoots rooted on half strength MS with 1.0 mg/l IBA + 1.0 mg/l NAA. For acclimation and transplantation the plants in the rooting culture vessels were kept in normal room temperature for seven days before transplan-ting in pots, where the plantlets were reared for three weeks. The survival rate of mature regenerants was found to be 75 - 80%.

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