Plant Tissue Cult. & Biotech. 15(2): 103-111, 2005 (December)

Micro-cloning in Commercially Important Six Bamboo Species for Mass Propagation and at a Large Scale Cultivation

S. A. M. Nurul Islam and Md. Mahbubur Rahman

Bangladesh Forest Research Institute, Sholoshahar, P.O. Amin Jute Mills, Chittagong-4000, Bangladesh. E-mail: nislam@abnetbd.com

Key words: Nodal bud, Cloning, Mini-clump, Proliferation, Cultivation

Abastract

In vitro cloning of some commercially important bamboos (viz. Bambusa balcooa, B. nutans, B. salarkhanii, B. vulgaris, B. vulgaris var. striata and Thyrsostachys oliveri) were tried by using nodal buds. Excised shoots were used as explants in transfer cultures and BAP (1 - 5 mg/l) was found to be effective growth regulator in liquid media for inducing multiple shoots (3 - 30). Half strength MS was supplemented with NAA (1 - 3 mg/l) and IBA (1 - 5 mg/l) for root induction. Two to three weeks old 3 - 5 cm long shoots in cultures were suitable for inducing rooting. Frequent changes of rooting media had improved production of rooted plantlets. Rooted plantlets were cultured in non-sterile water in the growth room for one week and after the plantlets were transferred to the propagation bed. The plantlets in propagation beds produced mini-clumps with 3 - 10 shoots within five - eight weeks. Rooted shoots of the mini-clumps were separated into two - five parts. The younger parts of mini-clumps were transplanted in the propagation bed for further proliferation of shoots; older parts were transferred in the soil-filled bags for field planting. These bagged plantlets were vigorous in growth and found suitable for large scale cultivation.

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