Plant Tissue Cult. 15(1): 57-65, 2005 (June)

Effect of Different Media on Callus Formation and Regeneration of Different Genotypes of Maize (Zea mays L.)

Muhammad Rafiq, Tahira Fatima, Tayyab Husnain, Khurram Bashir and Sheikh Riazuddin

National Center of Excellence in Molecular Biology (CEMB), University of The Punjab, Lahore, Pakistan

Key words: Regeneration, Different genotypes, Maize

Abastract

Immature zygotic embryos were harvested ten days after self-pollination from field-grown plants of four inbred lines and four synthetic varieties of maize (Zea mays L.) and cultured on five different callus induction media. The maximum callus formation ability for synthetic varieties (89 - 90%) and inbred lines (92 - 98%) was recorded in N6 medium (modified with 1.0 mg/l 2, 4-D; 1.0 mg/l 2, 4-D, 25 mM L-proline; 100 mg/l casein hydrolysate) in dark 25 ? 2?C, respectively. The study of interactions between media and different genotypes such as inbred lines FR-15, FD-2, CML-3, A-660 and synthetic varieties Agaiti-85, EV-1089, Golden and EV-5089 for callus formation and regeneration ability showed that FD-3 and EV-1089 appeared to be suitable candidates for plant transformation.

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