Rose, Rosa hybrida, Micropropagation, In vitro growth

Influence of Sucrose Concentration on in vitro Growth of Five Rose (Rosa hybrida L.) Cultivars

Nasser S. Al-Khalifah*, S. Hadi and F. Khan

King Abdulaziz City for Science and Technology, P.O. Box 6086, Riyadh-11442, Saudi Arabia

Key words: Plant Tissue Cult. 15(1): 43-49, 2005 (June)

Abastract

Influence of sucrose concentration of the medium, genotype and interaction of the two on in vitro growth responses of the cultures of five hybrid-tea rose (Rosa hybrida L.) cultivars were evident from the initiation of shoot proliferation from axillary buds on MS medium containing 4.0 mg/l BA, 0.2 mg/l Kn 1.0 mg/l GA3 and 30 g/l sucrose. Emerging shoots were subcultured to media containing 3.0 mg/l BA, 0.2 mg/l Kn and three different concentrations of sucrose (30, 40 and 50 g/l). Number of nascent shoots produced and elongation of the main shoot in a four-week-period was recorded for three consecutive subcultures in each concen-tration of sucrose. Rooting was induced on a medium containing 1.0 mg/l of IBA and respective sucrose levels. Sucrose concentration of 40 g/l produced the best response followed by 30 and 50 g/l. Genotypic influence was also apparent on growth responses with significant differences between values for shoots produced and shoot elongation in different cultivars. A significant interaction was observed between sucrose concentration and genotype. A negative correlation was found to exist between shoot proliferation and elongation. In general, Pristine White expressed the best growth response followed by Tropicana Orange, Peace Yellow, Paradise Pink and Oklahoma Red in that order.

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