Plant Tissue Cult. 15(1): 33-42, 2005 (June)

Regeneration Efficiency and Genotypic Effect of 15 Indica Type Bangladeshi Rice (Oryza sativa L.) Landraces

Mohammad Khalequzzaman*, Nazmul Haq, Md. Enamul Hoque and Tamal Lata Aditya

Department of Civil and Environmental Engineering, University of Southampton, Southampton, SO17 1BJ, UK

Key words: Mature embryos, Genotypic variability, Embryogenic callus, Plant regeneration, Indica rice

Abastract

Genotypic effect was observed for embryogenic callus induction and subsequent plant regeneration in 15 indica type Bangladeshi rice (Oryza sativa L.) landraces. Different growth regulators were found to influence callus induction ability and green plant regeneration. The highest frequency (55.6%) of shoot regeneration was observed in Hashikalmi with the supplementary hormone concentrations and combination of 2 mg/l BA, 1 mg/l NAA and 1.5 mg/l Kn in MS. The embryogenic calli derived from mature seeds successfully produced shoots, and rooted well on MS basal salt with IAA. Five landraces which could be utilized in further manipulation, such as transformation and in vitro hybridization, were found to have higher regeneration ability (? 50%). In vitro regenerated plants were successfully established in soil and produced fertile seeds.

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