Plant Tissue Cult. 15(1): 25-32, 2005 (June)

Direct In vitro Propagation of Asparagus adscendens Roxb.

Saurabh R. Mehta and R. B. Subramanian*

P. G. Department of Biosciences, Sardar Patel University, S.P.U. Campus, Vallabh Vidyanagar - 388 120, Gujarat, India

Key words: Asparagus adscendens, In vitro regeneration, Multiple shoots, Nodal explant, Rhizome extract

Abastract

A very high rate of multiple shoots was obtained from nodal explants of Asparagus adscendens Roxb. on MS supplemented with 0.27 mM NAA, 0.46 mM Kn and 0.6% agar. Good rooting response was observed when individual regenerated shoots were inoculated on to MS with 1.48 mM IBA, 3.90 mM ancymidol and 3% sucrose. Such plantlets were successfully transferred to soil after hardening with a high rate of survival. After six months, rhizomes of both in vivo mother plants and their in vitro regenerated counterparts were collected followed by their comparative phytochemical fingerprinting studies through HPTLC. The analysis did not reveal any phytochemical variation among the regenerants.

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