Plant Tissue Cult. 15(1): 1-6, 2005 (June)

Effect of Physical Factors on Micropropagation of Anthurium andreanum

Jaime A. Teixeira da Silva*, S. Nagae and Michio Tanaka

Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa, 761-0795, Japan

Key words: Acclimatization, Aeration, Rooting

Abastract

Anthurium shoot tips were successfully micropropagated on either agar- or rockwool-based substrates in either conventional glass bottles with (Milliseal? added) or without aeration, or within a Neoflon? PFA film Culture Pack?, with modified half strength of MS as the medium of choice. Rockwool proved to be a far better substrate than agar, while aeration, provided either by the presence of a Milliseal? on the cap or by the use of Neoflon? PFA film, enhanced the growth, rooting and subsequent acclimatization (100%) of this popular ornamental plant. A scaled-up model was then applied for the mass production (96 plants per vessel) of Anthurium with similar positive growth results.

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