Plant Tissue Cult. 14(2) : 167-172, 2004 (December)

In vitro Callus Induction and Plants from Stem and Petiole Explants of Salvia canariensis L.

Sebastiana Mederos-Molina

Department of Plant Physiology, Facultad de Farmacia, Universidad de La Laguna, Tenerife, Canary Island, Spain. E-mail: smedemol@ull.es

Key words: Salvia canariensis, Direct organogenesis, Callus, Stem, Petiole explants

Abastract

Stem and petiole explant of Salvia canariensis L. were cultured on MS and B5 culture media supplemented with BAP, NAA, IBA. Callus formation on the surface of stems and petioles and also high and low concentrations of NAA alone and combined with BAP, stimulated the callus induction and direct shoot formation, respectively. However, concentrations of IBA used here, inhibited shoot induction. The shoots developed from both explants formed roots when transferred to half strength MS medium supplemented with lAA, NAA or IBA.

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