Plant Tissue Cult. 14(2) : 161-166, 2004 (December)

Various Color Illumination Effect on In vitro Multiple Shoot Induction in Water Chestnut (Trapa japonica)

A. Hoque and S. Arima

Department of Agronomy and Agricultural Extension, University of Rajshahi, Rajshahi-6205, Bangladesh.

Key words: Color illumination, Multiple shoot, Induction, Water chestnut

Abastract

Shoot production efficiency through cotyledonary nodes as well as nodal explants were studied under various color illumination conditions in water chestnut (Trapa japonica Flerov). Under various color (white, blue, mixed, red and intra-red) illuminations, mixed color illumination showed the early and higher percentage of shoot proliferation. Fresh and dry weight were also significantly higher under the mixed color condition. Media consisted of MSMA with 2.7 ?M BA, 0.5 ?M NAA and 0.5 ?M GA3 in all color illuminations. The competence of cotyledonary node as well as nodal explants for the induction of multiple shoots under the influence of various color illumination as well as plant growth regulators have been observed. Shoots produced in vitro rooted 100% in liquid MS supplemented with 5.4 ?M IBA. Plantlets were established successfully in soil.

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