Plant Tissue Cult. 14(2) : 149-154, 2004 (December)

In vitro Plantlets Regeneration of Rose

Protul Kumar Roy, A. N. K. Mamun and Golam Ahmed

Plant Biotechnology and Genetic Engineering Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment (AERE), GPO Box-3787, Dhaka-l000, Bangladesh

Key words: Rosa sp., Plantlet regeneration, Nodal segments, Shoot tips

Abastract

Nodal segments and shoot tips were cultured on MS supplemented with different concentrations and combinations of BAP, NAA and zeatin for multiplying axillary shoots. Among two types of explants, the nodal explants produced the highest number of shoots when they were cultured on MS + 1.5 mg/l BAP + 0.25 mg/l zeatin + l00 mg/l CH. The number of shoots per explant in initial cultures varied from five to seven. But after three weeks when they were subcultured in the same medium, the number of shoots increased with an average of 15 per culture. Shoot tip explants also produced multiple shoots, but their performance was not as good as nodal explants. For best rooting, 34 cm long shoots were excised and implanted individually onto the rooting medium containing half strength MS fortified with 1.0 mg/l IBA and 0.5 mg/l IAA. Within four weeks of transfer to the rooting medium, 85% of the microcuttings produced seven - ten roots. The regenerated plantlets were successfully transferred to soil and the percentage of survivability under ex vitro condition was 80.

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