Plant Tissue Cult. 14(2) : 143-148, 2004 (December)

Method for Quality DNA Isolation from Different Parts of Jute Plant: Corchorus capsularis L. and C. olitorius L.

Samiul Haque, Nadim Ashraf, Aleya Awal, R. H. Sarker, Selina Begum and Haseena Khan

Department of Biochemistry and Molecular Biology, University of Dhaka,Dhaka-1000, Bangladesh

Key words: Jute, Corchorus capsularis, C. olitorius, DNA extraction, Polysaccharides, Metabolites

Abastract

A suitable method for isolating quality DNA from two cultivated species of jute (Corchorus capsularis and C. olitorius), which are rich in polysaccharides is described. Four types of plant materials such as seed, seedling, young leaf and old leaf from each species were used. Two isolation methods were followed with some modifications. The basic difference between the two methods was the presence or absence of phenol in the extraction protocol. The method lacking phenol failed to isolate DNA from seed samples of both species. The other method (using phenol) was capable of extracting DNA from all the samples but the quality of DNA was inferior to the other one.

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