Plant Tissue Cult. 14(2) : 113-121, 2004 (December)

Effect of Media Components on Nature of Callus and Subsequent Regeneration of Spring Wheat (Triticum aestivum L.)

M. Z. I. Talukder, A. K. M. Shamsuddin, H. Rahman and L. Hassan

Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh, Bangladesh

Key words: Callus nature, Media effect, Regeneration, Spring wheat

Abastract

The experiment was conducted to investigate the nature of callus and regeneration potential of immature embryos of wheat (Triticum aestivum L.) of five varieties: Protiva, Pavon, Kheri, Sonalika and Kanchan on MS supplemented with different concentrations and combinations of auxins and cytokinins. Calli developed from immature embryos by culturing them in MS with different concentrations of 2,4-D. There was a clear variation among the varieties and also among the different concentrations and combinations of auxin and cytokinin. The varieties Pavan and Protiva were found to be the best for producing good, compact and organized calli. MS containing 1.5 mg/l Kn gave good, compact and organized calli which turned yellow to brown over the period of incubation. Plant regeneration was best from compact, off-white, smaller calli although shoot regeneration was higher from calli of larger size.

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