Plant Tissue Cult. 14(1) : 55-61, 2004 (June)

Regeneration of an Indigenous Orchid, Vanda teres (Roxb.) Lindl. Through In vitro Culture

Pinaki Sinha and Shyamal K. Roy

Department of Botany, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh

Key words: Vanda teres, Indigenous orchid, Regeneration

Abastract

A protocol for regeneration of Vanda teres through long term culture of protocorms obtained from germinated seeds has been achieved in Vacin and Went medium. Seeds germinated within 40 - 45 days in Vacin and Went medium with 1.0 mg/l BAP + 0.5 mg/l NAA + 2% sucrose + 2 g/l peptone. Best shoot regeneration from the protocorm was found on the same medium containing 10% coconut water. For the production of strong elongated shoots and their multiplication 2 g/l banana powder + 100 mg/l casein hydrolysate were added to the medium. Identical plantlets with stout shoot and roots were obtained by subculturing shoots of same size in half strength MS medium without plant growth regulator. Enough quantity of regenerated plantlets were acclimatized in natural environment which flowered at the age of two - three years.

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