Plant Tissue Cult. 14(1) : 25-35, 2004 (June)

Submergence Tolerant Androgenic Rice Dihaploids Derived from Intra- and Interspecific Crosses: I. Seed Storage Protein and Isozyme Pattern Through Gel Electrophoresis

S. Mandal, N. Mandal, K. Hazra, S. K. Mukherjee and P. K. Das

Department of Biotechnology, Instrumentation & Environmental Science, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur-741 252, West Bengal, India.

Key words: SDS-PAGE, Isozyme, Submergence tolerance, Androgenic dihaploids, Rice

Abastract

Two cultivated indica rice Pankaj and Mahsuri susceptible to submergence were crossed with submergence tolerant semi wild FR-13A and wild O. rufipogon. FR-13A had better submergence tolerance than rufipogon as revealed by submergence tolerance score. F₁s of rufipogon exhibited higher cell abnormality in meiotic divisions and recorded high pollen sterility. F₁s of Pankaj x FR-13A and Pankaj x rufipogon exhibited higher level of submergence tolerance indicating crosses involving Pankaj with either FR-13A or rufipogon appeared to be better cross combinations. This would suggest that gene/s responsible for submergence tolerance are expressed more efficiently against the genetic background of Pankaj. Androgenic dihaploids were raised through anther culture from F₁s. Submergence tolerant androgenic dihaploid lines were identified through two passages of submergence tolerant screening test. They were evaluated for their seed protein polymorphism through SDS-PAGE as well for their esterase and peroxidase banding patterns. A general trend became evident where lower molecular wieght bands of either esterase or peroxidase as well seed protein similar to FR-13A and rufipogon were observed in submergence tolerant androgenic dihaploid lines. The dihaploid lines derived either from the crosses between Pankaj and FR-13A or between Pankaj and rufipogon displayed better survivability under submergence. Our results, therefore indicate that submergence tolerance favours the genetic background of Pankaj for its expression where FR-13A proves to be desirable donor for submergence trait.

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