Plant Tissue Cult. 14(1) : 9-15, 2004 (June)

Plant Regeneration in Physalis pubescens L. and Its Induced Mutant

Y. V. Rao, A. Ravi Shankar, T. V. R. Lakshmi and K. G. Raja Rao

Department of Botany, Andhra University, Visakhapatnam-530 003,

Key words: Physalis pubescens, Mutant, EMS, Regeneration

Abastract

Suitable protocols for callus induction and regeneration were developed for leaf and nodal segments of Physalis pubescens L. and its flowerless mutant. Per cent callus induction and regeneration were higher in the young leaves followed by mature leaves of the control and its mutant in that order in any combination of the media. MS medium containing 2.0 mg/l and 2.5 mg/l 2,4-D are the optimum concentration for induction of callus from leaf and stem segments, respectively. Optimum medium for callus induction of nodal explants is MS + 2.5 mg/l 2,4-D + 2.0 mg/l IAA. The per cent callus induction was higher in the explants of stem and node of the control over those of the respective organs of the mutant. Nevertheless MS basal medium supplemented with 2.0 mg/l BAP + 0.5 mg/l NAA is the optimum for regeneration of callus induced from leaf and nodal segments. Regeneration could not be obtained from the callus of stem of either control or mutant in any combination of MS medium. Probable role of the mutagen ethyl methane sulphonate (EMS) either in callus induction or regeneration of calli of the mutant is suggested.

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