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Plant Tissue Cult. 13(2) : 135-144, 2003 (December)

Changes in the Callus Soluble Proteins of Winter and Spring Wheat Cultivars following Cold Treatment

G. Karimzadeh1, K. Bagheri2 and M. Jalali-Javaran

Plant Breeding Department, College of Agriculture, Tarbiat Modarres University, P. O. Box 14115-336, Tehran, Iran

Key words: Triticum aestivum, Cold treatment, Embryogenic callus

Abastract

Quantitative changes in total soluble proteins were determined in the embryogenic callus derived from a spring (cv. Niknejad) and in a winter (cv. Sabalan) cultivars of bread wheat (Triticum aestivum L.) exposed to 4?C for two weeks. Mature embryos were excised and inoculated on MS supplemented with 2.0 mg/l 2,4-D, 0.2 mg/l BAP, 3% sucrose at pH 5.8 and solidified with 8 g/l purified agar. They were incubated in darkness at 25 ? 1?C for five weeks. Calli were subcultured on fresh medium and grown in the above mentioned conditions for 14 days. Half of the Petri dishes were transferred to 4 ? 1?C on day 14 for two weeks (cold treatment), and they were returned to 25?C. The other half was maintained at 25?C throughout the experiment (control treatment). Over the experimental period (14 - 40 days), calli were sampled at random every 48 h (3 X 0.5 g) for total fresh weight measurements. Total callus soluble proteins were extracted and their concentration was determined by a colorimetric method. Significant cold-induced increases in protein quantity occurred during the low temperature treatment, irrespective of the cultivar compared with the controls. Both wheat cultivars showed differences in their callus soluble proteins during the experimental period in response to temperature alterations.

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