Plant Tissue Cult. 13(2) : 117-123, 2003 (December)

In vitro Clonal Propagation of Paederia foetida L. - A Medicinal Plant of Bangladesh

M. N. Amin, M. M. Rahman and M. S. Manik

Department of Botany, University of Rajshahi. Rajshahi-6205, Bangladesh

Key words: In vitro, Clonal propagation, Paederia foetida

Abastract

Paederia foetida L. is an important perennial climbing medicinal herb. It becomes woody with age. Mass collection of the plant from natural habitats has led to its depletion. We have established a protocol for its mass propaga-tion that would help cultivation of this valuable plant in the homestead. Shoot tips and nodal segments from field-grown plants were used as explants and cultured on MS medium with 1.0 mg/l BA. Proliferation of shoots took place from axillary buds. Subculture of the nodal segments from initial culture on fresh medium gave a higher number of shoots. For rooting, shoots were excised from the proliferating cultures and implanted individually on root induction medium, consisting of half strength MS salts supplemented with 0.1 mg/l of IBA. Within five weeks of transfer, there was good rooting in 95% shoot cuttings. Plantlets were transferred to plastic pots maintained within a polythene tent. After two weeks of acclimatization they were transferred to the open environment where 80% of the plantlets survived.

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