Plant Tissue Cult. 13(2) : 109-116, 2003 (December)

Callus Induction and High-frequency Plant Regeneration of Pineapple (Ananas comosus (L.) Merr.)

M. Atique Akbar, Biplab K. Karmakar and Shyamal K. Roy¹

Department of Botany, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh

Key words: Pineapple, Callus, Regeneration, Acclimatization

Abastract

Meristem tips of the crown of Ananas comosus, cultured on MS supplemented with 1.5 mg/l NAA and 1.0 mg/l Kn, callused within three weeks. This callus, when subcultured on MS with 1.5 mg/l Kn + 0.5 mg/l NAA produced a large number of shoots. After four weeks, shoots were excised and implanted in shoot elongation medium consisting of MS basal salts fortified with 15% coconut water only. After harvesting shoots, the old callus was transferred to the fresh medium of the same constituents, where the old callus expanded and many new buds also emerged. Shoots rooted well within two weeks, when they were excised individually and implanted in half strength MS with 2.0 mg/l IBA. Eighty per cent plantlets survived when transferred to open field. Regenerated plants were morphologically uniform with normal leaf shape and growth pattern.

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