Plant Tissue Cult. 13(1) : 75-79, 2003 (June)

Plant Regeneration from Seed Derived Callus of three varieties of Basmati Rice

Hamid Rashid, Fida Mohammad Abbasi and Azra Quraishi

Agricultural Biotechnology Programme, Institute of Agricultural Biotechnology & Genetic Resources, National Agricultural Research Centre, Park Road, P.O. Box 45500, Islamabad, Pakistan

Key words: Oryza sativa, Callus, Regeneration, Somaclone

Abastract

Mature seeds of three rice varieties : Basmati 370, Basmati 385 and KS 282 were cultured on MS medium supplemented with 2.0 mg/l 2,4-D. The vareity KS 282 exhibited high callus induction efficiency (31.3%) followed by Basmati 385 (17.6%) and Basmati 370 (6.5%). Calli were maintained for 90 days by subculturing at 15 days interval on the same modified MS. Calli were transferred onto MS with different combinations of auxin and cytokinin. The highest freqeucny of plant regeneration was 71.42% for Basmati 370 and (57.14%) for Basmati 385 on MS supplemented with 0.5 mg/l NAA, 1.0 mg/l BAP. KS 282 showed the highest regeneration efficiency (75%) on the same medium but at lower concentration i.e., 0.4 mg/l NAA and 0.8 mg/l BAP.

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