Plant Tissue Cult. 13(1) : 53-59, 2003 (June)

Shoot Differentiation from Cotyledon Derived Callus of Chickpea (Cicer arietinum L.)

S. Huda, R. Islam, M. A. Bari and M. Asaduzzaman

Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh

Key words: Shoot differentation, Cotyledon, Callus, Cicer arietinum

Abastract

Regeneration of multiple shoots via callus induction and organogenesis was achieved from cotyledon explants of chickpea (Cicer arietinum L.). Callus induction and shoot regeneration at various frequencies were observed using different concentrations and combinations of growth regulators. Highest percentage (95) of callus formation was observed on MS + 3.0 mg/l 2,4-D + 3.0 mg/l BAP. The maximum percentage (40) of shoot bud formation was obtained on MS medium fortified with 2.0 mg/l BAP and 0.5 mg/l NAA with 2.50 number of shoots per callus. The regenerated shoots developed highest percentage (77) of roots on half strength of MS basal medium containing 1.0 mg/l IBA. Regenerated plants were successfully established in soil after acclimatization. Maximum survivability after four weeks of transplantation was achieved in 21 days old rooted shoots on soil.

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