Plant Tissue Cult. 13(1) : 21-29, 2003 (June)

Detection of Important Plant Viruses in In vitro Regenerated Potato Plants by Double Antibody Sandwich Method of ELISA

M. Salim Khan¹, M. I. Hoque², R. H. Sarker² and H.-P. Muehlbach

Department of Genetics, Institute of General Botany and Botanical Garden, University of Hamburg, Ohnhorststr, 18, D-22609 Hamburg, Germany

Key words: Potato, Solanum tuberosum, Virus detection, DAS-ELISA

Abastract

To develop a reliable and sensitive method for virus identification in vitro regenerated plantlets of six potato varieties such as, Cardinal, Diamant, Dhera, Multa, Cilena and Sieglinde were mechanically inoculated with seven potato viruses, namely A, Y, V, M, S, X and PLRV and identified through double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). In vitro regenerated non-inoculated plants were used as negative control. Among the seven viruses used, except PLRV others infected the host tissue mechanically. Although no physical symptoms could be observed in some cases after mechanical infection, the presence of viruses could be detected through DAS-ELISA test. There were no antiserum reactions in the non-infected and in vitro regenerated negative control plants.

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