Plant Tissue Cult. 12(2) : 195-213, 2002 (December)

Review Paper

Gene-chips in Plant Genetics

Narayana R. Isola*, A. Arundhati¹, T. V. Rajya Lakshmi¹ and A. Aruna Lakshmi¹

Oak Ridge Biotech (OrBit) Brentwood Circle, Oliver Springs, Tennessee 37840, USA. E-mail: nrisola@aol.com

Key words:

Abastract

Recent developments in the microarray technologies have contributed substantially to our understanding of gene regulation in plants. Availability of complete genome sequences coupled with the microarray methods allows a global comparison of the gene expression profiles and the development of a transctiptome. Transcriptome is a catalogue of all the expressed messages present in a cell (tissue). This approach, although in its development stages, has already proved to be invaluable in identifying new genes involved in signaling pathways as well as in confirming earlier findings in the elucidation of these pathways. This article is aimed at reviewing state-of-the-art of microarray techniques. A summary of the diverse applications of microarays in plant genetics is provided with suggestions for future research.

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