Plant Tissue Cult. 12(2) : 167-172, 2002 (December)

Regeneration of Plantlets Through Somatic Embryogenesis from Nucellus Tissue of Citrus macroptera Mont. Var. anammensis (?Sat Kara?)

M. N. Miah, Sahina Islam¹ and Syed Hadiuzzaman²

Institute of Life Sciences, National University, Gazipur, Bangladesh

Key words: Nucellus, Somatic embryogenesis, Plantlet regeneration, Citrus macroptera.

Abastract

Somatic embryogenesis and plantlet regeneration were achieved in callus cultures of nucellus derived from undeveloped ovules of immature fruits of Citrus macroptera. Four types of media were used but only modified MS medium supplemented with malt, responded well. Calli produced in malt-supplemented MS medium were embryogenic in nature. After transfer of embryogenic callus pieces in hormone-free MS medium, somatic embryos developed. Independent plantlets developed from these somatic embryos by further subcultures in the same medium. After five weeks of culture the plantlets were transferred to pot soil mixed with cow-dung-derived biogas slurry; they survived and grew well.

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