Plant Tissue Cult. 12(2) : 139-145, 2002 (December)

Plant Regeneration through In Vitro Cormel Formation from Callus Culture of Gladiolus primulinus Baker

Pinaki Sinha and Shyamal K. Roy

Department of Botany, Jahangirnagar University, Savar, Dhaka, Bangladesh

Key words: Gladiolus, Ornamental, Regeneration, Cormel formation

Abastract

Callus capable of plant regeneration was initiated at a higher frequency from slices of cormel sprouts of Gladiolus primulinus cv. Golden Wave, cultured in MS with 4.0 mg/1 NAA, 2.0 mg/1 BAP or 1.0 mg/1 2,4-D. Callus was maintained in callus initiation medium. Shoots regenerated from the callus in MS with 0.5-2.0 mg/1 Kn or/and 0.5-2.0 mg/1 BAP. The highest number of shoots regenerated in MS with 2.0 mg/1 BAP from one-month-old callus which was initiated in the medium with NAA. Shoot regeneration capacity of callus decreased with the age and after the fifth month no shoots were found to regenerate. The shoots produced roots in half strength MS with 2.0 mg/1 IBA and 6% sucrose. Rooted shoots produced cormels at the base of shoots in the same nutrient medium. About 80-90% cormels germinated, when planted in the field and 20% of them blossomed in the first year.

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