Plant Tissue Cult. 12(2) : 131-138, 2002 (December)

High Frequency Shoot Regeneration from Hypocotyl of Canola (Brassica napus L.) cv. Dunkled

Muhammad Ramzan Khan, Hamid Rashid¹ and Azra Quraishi

Agriculture Biotechnology Institute, National Agricultural Research Centre, Park Road, Islamabad, Pakistan

Key words: Brassica napus, Hypocotyl, Shoot regeneration

Abastract

Hypocotyl explants of Brassica napus L. cv. Dunkled were used to investigate the effects of various plant growth regulators and nitrate on morphogenesis in vitro. Two types of explants such as upper and lower portion of hypocotyls were examined. A high frequency of shoot regeneration (96%) was achieved on MS supplemented with growth regulators. Shoots regenerated earlier, if the explants were collected within three weeks from segments of the upper portion of hypocotyls, compared to those taken after three weeks from the lower portion, the shoot regeneration frequency remaining the same in both the cases. Exclusion of silver nitrate from the medium drastically reduced the regeneration potential (50%). Multiple shoot regeneration was obtained from the periodic subcultures of explants in the same medium. In vitro elongated shoots were rooted with a 100% success by using half-strength MS supplemented with 0.25 mg/1 IBA and 0.125 mg/1 IAA. Rooted plantlets were successfully established into the soil.

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