Plant Tissue Cult. 12(2) : 125-129, 2002 (December)

In Vitro Plant Regeneration from Cotyledonary Node of Psoralea corylifolia L.

M. Jeyakumar¹ and N. Jayabalan

Plant Tissue Cultur Unit, Department of Plant Science, School of Life Sciences,

Key words: Psoralea corylifolia, Cotyledonary node, Regeneration

Abastract

Cotyledonary node of Psoralea corylifolia L. gave rise to multiple shoots when cultured on MS medium supplemented with different concentrations of BAP and Kn. The highest rate of shoot multiplication was obtained MS containing 2.22 ?M BAP. The regeneration shootlets were rooted on MS basal medium with different concentrations of IBA. The maximum number of roots was produced on the medium containing 4.92 ?M of IBA. The plantlets, thus developed were hardened and successfully established in soil. Tissue culture raised plants exhibited normal growth, flowering and pod setting.

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