Plant Tissue Cult. 12(1) : 69-77, 2002 (June)

Regeneration and Agrobacterium-mediated Genetic Transformation of Two Indigenous Potato Varieties of Bangladesh

R. H. Sarker and Barkat Murtaja Mustafa

Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Key words: Micropropagation, Indigenous, Potato, Genetic transformation, Agrobacterium

Abastract

Maximum shoot regeneration in two indigenous potato (Solanum tuberosum L.) was observed on MS semi-solid medium supplemented with 1.0 mg/l BAP and 0.1 mg/l GA₃. Among the two varieties, namely Lal Pakri and Jam Alu the former showed best response in terms of number of shoots/explant, nodes/shoot and shoot length. Half strength of MS containing 0.1 mg/l IAA was found to be best for root induction from the excised shoots. Two strains of Agrobacterium namely, LBA4404 containing pBI121 and EHA105 having plasmid pCAMBIA1301 were used for transformation. Among the two strains, LBA4404 with the vector pBI121 showed better response. In the nodal segment of Lal Pakri, the transformed zone coincided with the regeneration point. Regeneration from transformed tissue was obtained on MS supplemented with 1.0 mg/l BAP and 0.1 mg/l GA₃. Histochemical GUS assay showed the expression of GUS gene in the leaf tissues of the transformed shoot.

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