Plant Tissue Cult. 12(1) : 57-68, 2002 (June)

Cloning and Expression Analysis of Two Endo-1,4-b-Xylanase Genes from Phanerochaete chrysosporium

Shakila Nargis Khan¹, Marufa Z. Akter and Paul F. G. Sims

Department of Biomolecular Sciences, University of Manchester, Institute of Science and Technology, P.O. Box 88, Manchester M60 1QD, United Kingdom

Key words: Xylanase, Phanerochaete chrysosporium, Cloning, Expression analysis

Abastract

The complete sequence analysis of two b-1,4-xylanase genes, xynA and xynB, from the model lignocellulose degrading white rot fungus Phanerochaete chrysosporium to the GenBank database (accession number AF301902 to AF301905 has been reported. Here we report the cloning and the successful expression of both genes to significant levels in a pET-based E. coli expression system. The size of the expressed proteins, XYNA and XYNB were approximately 48 and 37 kDa, respectively. In both cases, the vast majority of the expressed protein was located in the insoluble fraction presumably within the inclusion bodies. Finally, the presence of the recombinant proteins was confirmed by Western Blot analysis.

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