Plant Tissue Cult. 12(1) : 27-35, 2002 (June)

In vitro Rapid Clonal Propagation of Ocimum basilicum L.

F. Begum, M. N. Amin and M. A. K. Azad

Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh
E-mail: aminmn@Yahoo.com

Key words: Ocimum basilicum, Regeneration, Nodal segments, Shoot tip

Abastract

In vitro regenerated shoots of Ocimum basilicum L. were excised, sectioned into nodal pieces and subcultured individually in the nutrient medium which produced in an average of eight multiple shoots per transfer. For rooting, in vitro grown shoots were excised from the culture flask and implanted individually on root induction medium containing half strength of MS salts and 0.1 - 1.0 mg/l NAA, IBA or IAA. The highest frequency and healthy rooting was observed on MS medium containing 1.0 mg/l NAA. In vitro regenerated plantlets were transferred on to specially made plastic tray containing coco-peet as potting mix and thereafter successfully established under ex vitro condition. The survival percentage of transplanted plantlets was 75.

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