Plant Tissue Cult. 11(2) : 181-186, 2001 (December)

Comparative Tissue Culture Response of Wheat Cultivars and Evaluation of Regenerated Plant

Muhammad Farooq¹, Ihsanullah, Hamid Rashid*, Azra Quraishi and Khan Bahadar Marwat¹

Agricultural Biotechnology Institute, National Agricultural Research Centre, Islamabad, Park Road, Pakistan.
E-mail: abi_narc@yahoo.com

Key words: Triticum aestivum, Regeneration, Somaclonal variation

Abastract

Three wheat (Triticum aestivum L.) genotypes i.e. Bakhtawar-92, Punjab-96 and Inqilab-91 were tested for their response to callus induction frequency and their subsequent regeneration on a variety of media combinations. Bakhtawar-92 appeared to be the most responsive genotype to callus induction followed by Inqilab-91 and Punjab-96. It also produced significantly higher amount of callus as compared to other genotypes. However, the medium containing 2.0 mg/l 2,4-D was found to be optimum for callus induction irrespective of the genotypes. Regeneration frequency of Bakhtawar-92 was 40 per cent on the medium containing 0.1 mg/l IAA and 2.5 mg/l BAP. Punjab-96 and Inqilab-91 showed regeneration percentages of 25 and 33 on the medium supplemented with combination of 0.1 mg/l IAA and 0.5 mg/l BAP. Regenerated plants were evaluated for plant height, maturity and seed set. They showed favourable significant differences from the control plants at five per cent confidence level for most of the traits, plant height, days to maturity and kernels/spike etc.

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