Plant Tissue Cult. 11(2) : 151-157, 2001 (December)

Micropropagation of Mimosa pudica L. Through In vitro Culture and Using Gamma Irradiation

M. K. Munshi¹, S. N. Hossain, R. Islam, L. Hakim and M. Hossain

Plant Biotechnology and Genetic Engineering Division, Institute of Food and Radiation Biology (IFRB), Atomic Energy Research Establishment (AERE), G.P.O. Box 3787, Dhaka-1000, Bangladesh

Key words: Micropropagation, Mimosa pudica, Gamma irradiation

Abastract

Nodal segments and shoot tips obtained from Mimosa pudica L. were used for shoot multiplication via direct or indirect organogenesis. Maximum callus formation was obtained on MS medium in presence of 0.5 mg/l 2,4-D and 1.0 mg/l NAA when shoot tips were taken as explants. The maximum shoot regeneration from these calli was observed on MS medium containing 2.0 mg/l BA and 0.2 mg/l NAA. The highest frequency (92%) of direct multiple shoot formation was obtained from nodal segment on MS medium supplemented with 1.0 mg/l BA and 0.5 mg/l 2ip.Three-week-old culture was exposed to 5 - 30 Gy gamma rays. Fifteen Gy treatment enhanced the growth of shoots. Lower doses of gamma rays increased shoot length which declined at higher doses. The profuse rooting was observed upon transfer of individual microcuttings to half strength of MS supplemented with 0.5 mg/l IBA. In vitro grown plantlets were successfully transferred to pots.

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