Plant Tissue Cult. 11(2) : 133-140, 2001 (December)

In vitro Micropropagation of Poinsettia (Euphorbia pulcherrima Willd.)

Shyamal K. Roy and M. Ali Jinnah

Department of Botany, Jahangirnagar University, Savar, Dhaka, Bangladesh

Key words: Euphorbia pulcherrima, Shoot tip, Nodal segments, Micropropagation

Abastract

Multiple shoot buds were obtained from shoot tips and nodal explants of poinsettia (Euphorbia pulcherrima Willd.) on MS medium supplemented with 1.5 mg/l BA, 0.5 mg/l Kn and 15% coconut milk. Explants taken from in vitro proliferated shoots subsequently produced multiple shoots when cultured on the same nutrient medium. Repeated subculture resulted in rapid shoot multiplication at an average rate of 25 new shoot buds per subculture. The addition of 100 mg/l casein hydrolysate to the medium increased the length of shoots. In vitro raised shoots were rooted on half strength MS medium containing 1.0 mg/l IBA and 0.5 mg/l IAA. Following transplantation in the field 85 per cent of the regenerants survived and they blossomed uniformly after six months.

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