Plant Tissue Cult. 11(2) : 127-132, 2001 (December)

Determination of Optimal Bud Size for Higher Frequency of Microspore Derived Callus Induction and Regeneration in Two Cultivars of Mustard (Brassica juncea)

P. K. Saha and A. Roy¹

Department of Botany, Bose Institute, 93/1, Acharya Prafulla Chandra Road, Kolkata - 700 009, India.
E-mail: pksaha@bosemain.boseinst.ac.in

Key words: Brassica juncea, Bud size, Microspore, Regeneration

Abastract

In the cultivars RLM-198 and RJ-15 mustard (B. juncea) microspore formed callus was regenerated to form complete plantlets. Highest percentage of microspore calli was formed from floral buds pre-treated at 8 - 10⁰C for four days. Dosage of 2.0 mg/l either of 2,4-D or NAA in the culture medium elicited a higher response than other concentrations applied. Plant regeneration efficiency of calli induced in NAA supplemented medium was more. Rooting of shoots were induced in B5 medium without vitamins but supplemented with 0.5 mg/l NAA. Plantlets exhibited 32% haploid with 18 chromosome. After transplantation to pots 68% of plants survived. A correlation between different bud sizes and callusing response was recorded.

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