Plant Tissue Cult. 3(2) : 101-106, 1993

In Vitro Regeneration of Multiple Shoots in Three Bamboo Species

Ratan Lal Banik, S.A.M. Nurul Islam and Syed Hadiuzzaman*

Bangladesh Forest Research Institute, Chittagong, Bangladesh

Key words: Bamboo, Tissue culture, Multiple shoots

Abastract

Plantlets of Bambusa arundinacea, Dendrocalamus brandisii and Thyrsostachys siamensis have been developed by culturing excised plumules in in vitro condition. Production of 20-75 shoots was possible by repeated transfer of cultures at 3-4 weeks? interval either in liquid or on gelled MS or B5 medium supplemented with 1.0 mg/1 BAP, the gelled medium being found better than the liquid medium. For B. arundinacea and T. siamensis, 1.0 mg/1 BAP was optimum whereas higher concentration of BAP was required for D. brandisii. Healthy roots were also produced in the same medium within 6-7 weeks after the proliferation of shoots. A large number of plantlets have been successfully transferred from aseptic condition to the outdoor nursery; they are growing satisfactorily in the field.

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