Plant Tissue Cult. 11(1) : 85-91, 2001(June)

In vitro Propagation of Chrysanthemum (Chrysanthemum morifolium Ramat) Through Callus Culture

R. H. Sarker and Ismat Shaheen

Department of botany, University of Dhaka, Dhaka-1000, Bangladesh
E-mail: baptc@bd.drik.net

Key words: Propagation, Chrysanthemum, Callus culture, Leaf explant

Abastract

Using leaf explants with an intervening callus phase a suitable protocol was developed for in vitro regeneration of Chrysanthemum morifolium Ramat var. ?Snow ball?. The best response for callus induction and consequent development of shoots was observed when 1.5-2.0 cm long leaf explants were cultured on MS medium containing 5.0 mg/1 BAP and 0.5 mg/1 Kn. The maximum number of shoots from the callus developed along the entire surface of the leaf. Shoots also regenerated from mid rib callus on MS medium fortified with the similar hormonal supplements but comparatively with a less frequency. Roots developed (100%) on MS medium containing 0.2 mg/1 IBA. Plantlets were successfully established is soil. Anatomical studies on freezing microtome sections revealed the formation of several shoot buds as well as organization of vascular tissue within the developing shoots.

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