Plant Tissue Cult. 10(2) : 143-148, 2000 (December)

Establishment of a High Frequency Shoot Regeneration Method from Cotyledon Explants in Jute (Corchorus capsularis L. var. D-154)

M. Khalekuzzaman¹, Y. Takagi, R. Islam¹, M. F. Alam¹ and M. Hossain¹

Laboratory of Plant Breeding, Faculty of Agriculture, Saga University, Saga-840, Japan

Key words: Corchorus capsularis, Cotyledon, Petiol, Shoot bud formation, Plant regeneration

Abastract

An efficient tissue culture method for a high rate of shoot regeneration was developed for Corchorus capsularis L. var. D-154 (jute). Cotyledonary explants with and without petiol were cultured in MS medium supplemented with auxin and cytokinin. Explants with the petiol showed a better response than those without it in respect of shoot initiation. The best shoot proliferation was observed when cotyledon-derived calli was cubcultured in medium fortified with a combination of 2.0 mg/1 BA and 0.5 mg/1 IAA. In vitro elongated shoots were rooted with a 100% success by treating them with a 0.3 mg/1 IBA. Rooted plantlets were successfully established into soil and the survival rate of the plants was more than 80%. The regenerants matured as normal and produced fertile seeds.

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