Plant Tissue Cult. 3(2) : 91-96, 1993

Micropropagation of Dianthus barbatus and D. chinensis through Cotyledonary node Culture

Vidya Jethwani and S.L. Kothari

Department of Botany, University of Rajasthan, Jaipur, India

Key words: Micropropagation, Cotyledonary node, Dianthus barbatus, D. chinensis

Abastract

Cotyledonary node explants (2-3 mm) of Dianthus barbatus and D. chinensis were cultured on B5, LS, MS and MSB basal media supplemented with BAP and NAA. The best response in terms of multiple shoot formation was observed on LS medium with 2.0 mg/1 BAP and 0.50 mg/1 NAA. D. barbatus showed a higher frequency of shoot formation as compared to D. chinensis. In six weeks over 100 shoots were observed in D. barbatus and 60-65 in D. chinensis. Every two weeks inoculi of 6-8 shoots were transferred onto fresh medium for further shoot differentiation. Shoots at every subculture were rooted on hormone-free medium. Rooted plants were transferred to soil where they flowered normally and resembled seed-grown plants.

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