Plant Tissue Cult. 10(2) : 125-130, 2000 (December)

Micropropagation of Patal (Trichosanthes dioica Roxb.) from Nodal Segment and Shoot Tip

Ripon Kumar Debnath, Shyamal Kumar Roy, Golam Ahmed¹ and Mamun Hossain¹

Plant Tissue Culture Laboratory, Department of Botany, Jahangirnagar University, Savar, Dhaka, Bangladesh

Key words: Micropropagation, Trichosanthes dioica, Nodal segment, Shoot tip

Abastract

An efficient protocol was developed for direct plant regeneration, multiplication and rooting in in vitro condition of Trichosanthes dioica locally known as ?patal?. Shoot tips and nodal segments from field grown plants were culture aseptically on MS supplemented with different concentrations and combinations of cytokinins (BAP and Kn) and auxins (NAA, IBA and IAA) for direct shoot regeneration. Effects of coconut milk and adenine sulphate were also evaluated. The frequency of multiple shoot regeneration response was 92 and 100%, for shoot tips and nodal segments, respectively when cultured on the medium containing MS + 2 mg/1 BAP + 0.1 mg/1 NAA. Average number of shoots per explant was 24 and 26 for shoot tips and nodal segments, respectively. The length of the longest shoot was 8.6 and 8.9 cm, respectively, for shoot tips and nodal segments cultured on MS medium supplemented with 1.0 mg/1 BAP + 0.1 mg/1 NAA + 10 mg/1 adenine sulphate (AS). Efficient rooting was achieved on half strength of MS + 0.5 mg/1 NAA. In vitro raised plantlets were transferred to potted soil and finally transferred to the experimental field.

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