Plant Tissue Cult. 10(2) : 119-124, 2000 (December)

Magnitude of Somaclonal Variation in Oryza sativa L. cvs. Basmati-385, JP-5, Pakhal and Swat-II

Naheed Gul, Zahoor A. Swati¹, S. M. Saqlan Naqvi, Ihsan Ullah and Azra Quraishi²

Agricultural Biotechnology Institute, National Agricultural Research Centre, Park Road, Islamabad, Pakistan

Key words: Magnitude, Somaclonal variation, Oryza sativa

Abastract

In vitro callus formation and plant regeneration response of four rice cultvars including Basmati-385, JP-5, Pakhal and Swat-II to different culture media were studied. Two culture media one without harmones and the other with 2 mg/1 of 2,4-D and 0.2 mg/1 of kinetin were used for callus induction. Medium supplemented with growth hormones gave good results. High callus formation was observed in Pakhal (70%) and Basmati-385 (58%). For regeneration, two modified MS media, MSK₂ with 2 mg/1 of Kn and 0.2 mg/1 IAA and MSK₅ with 5 mg/1 Kn and 0.5 mg/1 of IAA were tested. Mean regeneration efficiency of all the genotypes on MSK₅ medium was higher than that of MSK₂. Genotypes were significantly different in their response to regeneration. Highest mean regeneration efficiency was recorded in JP-5 (64%) followed by Swat-II (51%), Pakhal (44%) and Basmati-385 (30%). All plants derived from tissue culture showed desirable in all the agronomic traits studied except 1000 grain weight.

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