Plant Tissue Cult. 10(2) : 93-102, 2000 (December)

Influence of Different Plant Growth Regulators on In vitro Multiplication and Rooting of ?Nabali? olive (Olea europea L.)

Rida A. Shibli¹, Abdullah Abu-Ein and Karim H. Al-Juboory²

Biotechnology Center-Agriculture, Jordan University of Science and Technology, Irbid, Jordan

Key words: Growth regulators, Olea europea, Shoot multiplication, Rooting

Abastract

Olive (Olea europea L.) is a long-lived evergreen tree that is adapted to many soil types and environmental conditions. To our knowledge, there is no olive cultivar which is better adapted to water and salinity stress more than ?Nabali?. Nurserymen neglected this cultivar because its cuttings are low in rooting capacity; as a result this cultivar is no longer available in abundance for planting. In order to meet this shortage in the supply of ?Nabali? successful in vitro multiplication and rooting protocols have been developed for ?Nabali?. A higher concentration (5.0 mM) of both benzyl adenine (BA) and kinetin (Kn) significantly increased the number of proliferated shoots per explant up to 4.5. A higher concentration of BA, zeatin and Kn induced more callusing and hyperhydrated microshoots. 2ip was less effective in shoot proliferation. Among different auxins IAA, IBA and NAA were tested at 0.0, 5, 10 and 15 ?M concentrations;a maximum of 75% rooting was achieved at 10 ?M IAA with 3.5 roots/explant. A 0.2 g/1 polyvivylpyrrolidine (PVP) was used in media of different experiments to eliminate phenolic exudates. Rooted microshoots were successfully acclimatized ex vitro under intermittent misting with 90% survival rate.

Download Full Article