Plant Tissue Cult. 10(1) : 51-58, 2000 (June)

Adventitious Shoot Bud Formation from Cultured Leaf Explants of Rheum emodi Wall.

Nand Lal¹ and P. S. Ahuja

Central Institute of Medicinal & Aromatic Plants (CIMAP), Post Box No. 1, RSM Nagar, Lucknow-16, India

Key words: Adventitious shoot buds, Leaf explants, Rheum emodi

Abastract

The optimal adventitious shoot bud induction (4.33 ? 0.56) in leaf explants of Rheum emodi Wall. and their subsequent growth were obtained on MS medium supplemented with BAP (2.0 mg/1) and IBA (1.0 mg/1) within six weeks. Although initially BAP induced shoot buds in leaf explants their subsequent growth into separable shoots required transfer of regenerating leaves on to hormone-free MS medium for a period of three-four weeks. Comparison of different leaf portions revealed that shoot bud regeneration potential was confined only to intact whole leaves, basal half lamina and basal part of petiole. Epidermal layers and leaf tissue without epidermal layers failed to show bud regeneration response. Histological and SEM studies showed no evidence of somatic embryogenesis during bud regeneration.

Download Full Article