Plant Tissue Cult. 10(1) : 17-23, 2000 (June)

In vitro Propagation of Thankuni (Centella asiatica L.)

S. N. Hossain, S. Rahman, A. Joydhar, S. Islam and M. Hossain¹

Biotechnology Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Savar, G.P.O. Box 3787, Dhaka-1000, Bangladesh

Key words: Thankuni, Centella asiatica, In vitro propagation

Abastract

Stem node explants of naturally grown Centella asiatica L. were used for in vitro regeneration of multiple shoots. Various combinations of BAP and NAA in different concentrations were used in the regeneration of multiple shoots and a concentration of 1.0 mg/1 BAP and 0.5 mg/1 NAA was found superior in the optimum production of multiple shoots. Among the three auxins used in different concentrations, 0.2 mg/1 IBA was found effective in the production of roots. Eighty per cent of the plantlets produced from in vitro culture method survived in the ex vitro condition. Measurement of chlorophyll a, chlorophyll b, carotenoid and soluble protein content of fresh leaf of both in vitro regenerated and natural plant represented non significant defferences.

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