Plant Tissue Cult. 1 (2) : 67-73, 1991

EFFECT OF GAMMA RADIATION ON CALLUS GROWTH AND PLANT REGENERATION IN CORCHORUS CAPSULARIS JUTE

G. Ahmed and M. S. Islam

Genetics Division, Institute of Food and Radiation Biology, Bangladesh Atomic Energy Commission, A. E. R. E., G. P. O Box 3787, Dhaka, Bangladesh

Key words:

Abastract

Seeds of C.capsularis var. D-154 were irradiated with different doses ranging from 0 to 800 Gy (with 100 Gy interval) delivered at the dose rates of 40, 130 and 390 Gy/hr. Cotyledons were cultured on MS medium + 0.5 mg/1 BAP + 1 mg/1 2, 4-D for initiation and growth of callus. Plumules with decapitated roots were cultured on MS + 0.5 mg/1 BAP + 25 mg/1 tyrosin to induce multiple shoot formation. With the increase of exposure dose, there was gradual reduction in the growth of calli. The percentage of shoot forming explants and the number of shoots per explant were decreased as the exposure dose increased. Moreover, the increase in exposure dose delayed callus and shoot bud formation and also inhibited the development of shoot buds into vigorous shoots. At the same dose, the higher dose rate (390 Gy/hr) proved more detrimental for multiple shoot formation than the lower ones (40 and 130 Gy/hr). But no such effect was observed on the growth of calli.

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