Plant Tissue Cult. 9(2) : 177-180, 1999 (December)

Short Communications

In vitro Organogenesis of Various Explants of Azadirachta indica A. Juss (Neem)

M. N. Abubachker¹ and S. Alagumanian

P. G. Department of Botany, National College, Tiruchirappalli-620 024, India

Key words: Callus, Organogenesis, Azadirachta indica

Abastract

Azadirachta indica A. Juss is commonly known as neem tree belonging to the family Meliaceae. The neem tree is an indigenous species of South East Asia. The plant possesses several medicinal, insecticidal and biopesticidal properties (Dreyer and Hellpapa 1992, Ahmed 1993, Saeed 1993). Neem plants are generally propagated through seeds. But neem seeds usually lose their viability within two to three weeks of their maturity (Ramaswamy 1993). Therefore, several studies have centered on A. indica callus induction and organogenesis from leaf, stem, anther and embryo culture (Gautam et al.1981, Ramesh and Padhya 1988). The aim of the present study is to know the role of growth regulators in various concentrations and combinations (auxin 2,4-D and IAA, cytokinin, BA) on A. indica in vitro organogenesis.

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