Plant Tissue Cult. 9(2) : 159-166, 1999 (December)

An Effective Method for In vitro Production of Disease-free Carnation (Dianthus caryophyllus) cv. Balady

Rida A. Shibli¹, Mohmmed M. Ajlouni, Mohamad A. Shatnawi and Abdullah Abu-Ein

Biotechnology Center, Jordan University of Science and Technology, P.O. Box 3030, Irbid, Jordan

Key words: Carnation, Micropropagation, Rooting, Shoot multiplication

Abastract

An efficient procedure for production of disease-free carnation (Dianthus caryophyllus) cv. ?Balady? on MS medium was developed. Highest shoot formation was obtained on medium supplemented with 1.0 mg/1 6-benzylaminopurine (BA) and 0.1 mg/1 1-naphthalenacetic acid (NAA). Maximum rooting was achieved on medium containing 1.0 mg/1 indole-3-butyric acid (IBA) with 0.1 g/1 activated charcoal (AC) or 0.1 g/1 phloroglucinol (PG). Rooted plantlets were transferred to greenhouse in 1 peat : 1 perlite mixture and grew normally. Ex vitro rooting was successfully achieved when microcuttings basal ends were dipped for 3 sec in 0.5 g/1 IBA and planted in 1 peat : 1 perlite mixture. Flowers of tissue culture derived plants did not show any phenotypic variation from those produced by direct in vitro rooting of stem cutting.

Download Full Article