Plant Tissue Cult. 9(2) : 141-150, 1999 (December)

In vitro Morphogenesis in Chickpea (Cicer arietinum L.)

R. H. Sarker and Shaikh Tahmina Awal

Department of botany, University of Dhaka, Dhaka-1000, Bangladesh

Key words: In vitro, Morphogenesis, Cicer arietinum

Abastract

Culture conditions were developed for in vitro plant regeneration with or without the intervening callus phase from two locally grown varieties of chickpea (Cicer arietinum L.) namely, Binasola-2 and Hyprosola. The best response regarding callus induction obtained when immature embryo was cultured on MS medium containing 3.0 mg/1 BAP and 0.5 mg/1 NAA in both Binasola-2 and Hyprosola. Maximum number of shoots obtained when immature embryo derived callus was cultured on MS medium containing 5.0 mg/1 BAP + 0.5 mg/1 Kn and only 5.0 mg/1 BAP in Binasola-2 and Hyprosola, respectively. Highest frequency of direct shoot morphogenesis without the intervention of callus was achieved from the cotyledonary node and surrounding tissue on MS medium containing 0.25 mg/1 BAP + 0.1 mg/1 Kn for both the varieties. Root induction at the base of the isolated regenerated shoots was found to be the best when they were cultured on full strength of MS medium containing 0.05 mg/1 IBA. Histological and histochemical studies through freezing microtome revealed the formation of several shoot buds from the peripheral region of callus tissues and development of shoot apex as well as the organization of vascular tissue within the developing shoots. Such studies also demonstrated the organizational features during the formation of roots.

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