Plant Tissue Cult. 9(2) : 133-140, 1999 (December)

In vitro Micropropagation of Cymbidium aloifolium L. SW. and Spathoglottis plicata Bl.

A. K. Barua and S. K. Bhadra

Department of botany, University of Chittagong, Chittagong-4331, Bangladesh

Key words: Micropropagation, Cymbidium aloifolium, Spathoglottis plicata

Abastract

The shoot and leaf segments of in vitro germinated seedlings of Cymbidium aloifolium and Spathoglottis plicata were aseptically grown on 0.8% (w/v) agar solidified three different media, namely Murashige and Skoog (MS), Phytamax (PM) and Modified Vacin and went (MVW) supplemented with 2-3% (w/v) sucrose and different combinations of auxins (IAA, IBA, NAA and 2,4-D) and cytokinin (BAP and zeatin) at different concentrations. The shoot segments having nodal zone of both the species produced multiple shoot buds on all the media with different combinations of hormones. Leaf segments, however, did not give any response to any of these media. In case of C. aloifolium the maximum number of multiple shoot buds/shoot segment was developed on MVW supplemented with 2% (w/v) sucrose + 0.05 mg/1 IAA + 2.0 mg/1 zeatin. On the other hand, I case of S. plicata MS medium supplemented with 3% (w/v) sucrose + 0.1 mg/1 IBA + 2.0 mg/1 BAP was proved best. The multiple shoot buds of both the species underwent rapid elongation on the same media but did not produce better root system. In order to induce stout and strong root system, the shoot buds at a stage of 2-3 cm length were individually cultured on two different 0.8% (w/v) agar solidified media, namely (i) half strength of MS + 1.5% (w/v) sucrose and (ii) MS + 3% (w/v) sucrose + 0.5 mg/1 IAA. The former medium was better than the latter in both the species. These in vitro grown seedlings were then successfully transferred to outside natural condition through successive phase of acclimatization.

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