Plant Tissue Cult. 9(2) : 127-132, 1999 (December)

Ex vitro Survival of In vitro Derived Banana Plants Without Greenhouse Facilities

Y. T. Jasrai¹, V. R. Kannan, A. Remakanthan and M. M. George

Department of botany, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara-390 002, India

Key words: Banana, Acclimatization, Micropropagation, Shoot tip

Abastract

Clonal propagation of banana was achieved from shoot tip explants isolated from suckers of elite plants. Six to seven micro-shoots were produced from the basal lateral sides of the main shoot tip, when cultured on modified MS medium supplemented with 6-benzyladenine (BA). Induction of roots was achieved on micro-shoots when cultured on MS medium supplemented with indole-3-acetic acid (IAA). The well developed plantlets were hardened without greenhouse facilities for their establishment in the field. On an average 92% of the plantlets survived hardening with the protocol developed.

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