Plant Tissue Cult. 9(2) : 107-111, 1999 (December)

Shoot Regeneration in Chickpea (Cicer arietinum L.) from Callus Cultures of Internode Explants

S. Huda, R. Islam and M. A. Bari

Institute of Biological Science, University of Rajshahi, Rajshahi-6205, Bangladesh

Key words: Chickpea, Internode explants, Regeneration, Organogenesis

Abastract

Regeneration of multiple shoots via callus induction and organogenesis were achieved in chickpea (Cicer arietinum L.). Somatic embryos were obtained from internode derived callus of chickpea. For obtaining a high frequency of embryogenic cultures, growth regulator supplement and incubation conditions during the callusing phase are critical. B5 medium supplemented with 3.0 mg/1 2-4,D and 3.0 mg/1 BAP, 3.0 mg/1 NAA and 3.0 mg/1 BAP and dark incubation was found best for producing organogenic callus. Development of adventitious shoots occurred when the calli were subcultured in MS medium supplemented with IAA and Kn. Maximum frequency of calli (60%) induced adventitious shoots and highest number of shoots per callus were obtained when the medium was fortified with 2.0 mg/1 Kn and 2.0 mg/1 IAA.

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