Plant Tissue Cult. 9(1) : 53-61, 1999 (June)

Regeneration of Plantlets In vitro from the Seedling Explants of Pomegranate (Punica granatum)

M. N. Amin, M. N. Islam and M. A. K. Azad

Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh

Key words: In vitro multiplication, Seedling explants, Punica granatum

Abastract

Cotyledon and hypocotyl explants were collected from the in vitro grown seedlings and cultured on half-strength of MS medium for inducing callus and proliferation of adventitious shoots. The cultured explants produced callus from their cut margins and supplemently adventitious shoots on half-strength of MS medium supplemented with 0.5-0.2 mg/1 BA and 0.1-0.5 mg/1 NAA, IBA or IAA. Maximum frequency of cultures produced shoots and the highest number of shoots per culture were observed when the half-strength of MS medium was amended with 1.0 mg/1 BA and 0.1 mg/1 NAA. For rooting, the in vitro proliferated and implanted individually on root induction medium containing half-strength of MS medium supplemented with 0.5 mg/1 NAA. Within three weeks of transfer to the rooting medium 100% of the cultured microcuttings produced 2-5 roots in this medium. The rooted shoots, regenerated plantlets, were gradually acclimatized and successfully established on the soil under natural condition with about 75% survival.

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